REVUE ALGERIENNE D’ALLERGOLOGIE
Volume 10, Numéro 2, Pages 51-58
2026-01-05

The Contribution Of Molecular Allergy Diagnostics To Identifying Cross-reactive Allergies Involving Profilin And Nsltp : A Case Report

Authors : Boussaha Hadia . Lammara Mahammed Lydia . Gharnaouet Merzak . Djidjik Réda .

Abstract

Background: The advent of molecular diagnostics, particularly Component Resolved Diagnosis (CRD), has been a significant advancement in the understanding of the underlying mechanisms of allergies. CRD is based on the identification of specific molecular allergens. Purpose: This case aims to illustrate the value of molecular diagnostics in identifying co-sensitization and cross-reactivity profiles, as well as to discuss the practical implications for management, particularly in terms of prevention and patient education. Methods: This report describes the case of a 25-year-old Algerian patient with pollen-food allergy syndrome, as well as co-sensitization to profilin and nsLTP. Results : The patient presented with a history of allergic rhinitis and conjunctivitis during the spring. He reported experiencing dyspnea and urticaria immediately after ingesting peanuts and nuts, though sometimes he had no symptoms. He also experienced oral allergy syndrome, immediately after eating certain fruits, such as apples, peaches, and tomatoes. The results of specific IgE antibodies for allergen extracts indicated that the patient had a very broad sensitization profile. Subsequently, molecular allergen testing was performed using a customized allergen-chip based on the ISAC technology. Specific IgE against molecular components were positive, with most antibodies belonging to two molecular families: profilins and nsLTP. Profilin is often associated with moderate allergic reactions. In contrast, nsLTPs proteins are linked to more severe clinical manifestations. Co-sensitization to Profilins and nsLTP can modulate clinical severity. Additionally, co-factors strongly influence the variable clinical expression of nsLTPs. Conclusion: This case clearly illustrates the importance of an allergen-specific diagnosis, based on allergenic molecules (CRD-component-resolved diagnosis), for identifying cross- and co-sensitization to multiple allergens. This approach allows for personalized, accurate diagnoses and optimized management of allergic diseases.

Keywords

CRD ; Co-sensitization ; Profilin ; nsLTP ; Molecular Allergy Diagnostics