Journal of Molecular and Pharmaceutical Sciences
Volume 3, Numéro 2, Pages 21-32
2025-04-10
Glue Thistle Toxicology: In Vitro Assessment Of Oxidative Stress Induced By The Active Compound Atractyloside In Atractylis Gummifera L
Authors : Belmahi Mohamed.habib . Mecheri Imane . Chader Henni .
Abstract
Glue Thistle, scientifically known as Atractylis gummifera L., is commonly used in traditional medicine due to its topical wound-healing properties for conditions like syphilis, boils and abscesses. Additionally, it serves as an herbal infusion with diuretic, purgative, antipyretic, abortifacient and emetic qualities. The acute toxicity of this plant varies across animal species and administration routes, typically leading to hepatic necrosis and renal failure. The primary aim of our study is to assess the toxic potential of atractyloside, the primary active compound in Glue Thistle, in vitro. This is achieved by determining the inhibitory concentration 50 (IC50) on three different cell lines (RD, HEP2, and A549) and evaluating oxidative stress indicators, i.e. specifically, reduced glutathione (GSH), malondialdehyde (MDA) and catalase, in these cellular models. In vitro results indicate a significant increase in MDA levels, a decrease in reduced GSH concentration, resulting in a reduction in catalase activity ranging from 66% to 85%. These outcomes suggest oxidative stress induced by atractyloside, accompanied by an apoptotic process. Our research contributes to a comprehensive understanding of the acute toxicity of Atractylis gummifera L., offering insights for more effective preventive and treatment approaches.
Keywords
Atractylis gummifera L ; Glue Thistle ; Atractyloside ; In vitro ; Oxidative stress ; Toxicity
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