Global Research in Applied Cosmetology and Esthetics
Volume 0, Numéro 0, Pages 04-11
2026-09-01
Valorization Of Olive Leaves: Comparative Assessment Of Extraction Techniques And Cultivar Effects On Polyphenol Content And Antioxidant Potential Of Algerian Olive Leaves (olea Europaea L.)
Authors : Bennara Karima . Missaoui Racha . Nabti Bachir . Boudis Abdelhakim .
Abstract
Olive leaves are underexploited yet rich sources of bioactive phenolics such as oleuropein, with growing interest for dermocosmetic, nutraceutical, and therapeutic applications. Extraction efficiency and antioxidant activity are highly dependent on both the extraction method and the cultivar's geographic origin. This study compared three conventional extraction techniques across diverse Algerian olive cultivars to identify optimal cultivar–method pairings for bioactive recovery. Leaves from four samples—Chemlal (Jijel and Médéa), Sigoise (Jijel), and a mixed Batna sample (Chemlal, Sigoise, Tefah)—were subjected to ultrasound-assisted extraction (UAE, 47% ethanol, 35 kHz), Soxhlet extraction (60% ethanol), and maceration (70% ethanol). Extracts were evaluated for yield (dry extract), total phenolic content (TPC, Folin–Ciocalteu method), and antioxidant capacity via DPPH and ABTS assays (IC50). Soxhlet yielded the highest biomass (22.21–43.81%), notably from Médéa Chemlal (43.81%), but often produced viscous, resin-rich extracts. UAE resulted in lower yields (6.47–27.77%) but higher antioxidant efficiency in coastal cultivars. Sigoise–UAE extracts exhibited the strongest DPPH activity (IC50 = 6.34 ± 1.27 µg/mL), while Chemlal–UAE (Jijel) showed the highest TPC (159.89 ± 14.95 µg GAE/mg). ABTS performance favored Chemlal–Soxhlet (IC50 = 1.80 ± 0.66 µg/mL). UAE is ideal for phenolic-rich extracts from coastal varieties, while Soxhlet maximizes recovery from resinous, arid-region cultivars. These insights support targeted valorization of olive leaves for sustainable, plant-based antioxidant formulations in dermocosmetic and health-related sectors.
Keywords
Olea europaea; Olive leaves; phenolic antioxidants; extraction optimization; cultivar specificity; underutilized biomass
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