Batna Journal of Medical Sciences
Volume 13, Numéro 8, Pages 625-630
2026-07-30

Formulation Of A Root Canal Disinfectant Solution Based On Essential Oils Of Origanum Vulgare And Rosmarinus Officinalis Against Enterococcus Faecalis



Authors : Himeur Besma . Bousselham Ammara .

Abstract

Introduction Endodontic infection, caused by microorganisms such as Enterococcus faecalis, represents a major clinical challenge. This bacterium is resistant to traditional disinfectant solutions like sodium hypochlorite and chlorhexidine. Therefore, it is crucial to explore more effective and less toxic alternatives. The objective of this study was to assess the antimicrobial effectiveness of essential oils from Origanum vulgare and Rosmarinus officinalis as natural alternatives against Enterococcus faecalis. Materials and Methods The essential oils of Origanum vulgare and Rosmarinus officinalis were extracted by hydrodistillation. Their antimicrobial activity was measured using two methods: disc diffusion and microdilution to determine the minimum inhibitory concentration (MIC). Solutions of essential oils were prepared by combining the oils with a solubilizer and distilled water to evaluate their effectiveness. Results The yield of essential oils was 1.86% for Origanum vulgare and 0.87% for Rosmarinus officinalis. Origanum vulgare oil showed significant antibacterial activity, with an inhibition zone diameter of 30 mm and an MIC of 2.78 mg/ml. In contrast, Rosmarinus officinalis oil exhibited weak activity, with an inhibition zone diameter of 8 mm and an MIC of 11 mg/ml. The combination of the two oils showed no additional benefit. Discussion The results indicate that Origanum vulgare essential oil could be an effective alternative to traditional disinfectant solutions. However, Rosmarinus officinalis oil did not show satisfactory results. The combination of both oils does not provide any significant improvement. Therefore, Origanum vulgare oil offers an interesting potential to replace conventional disinfectants in the treatment of endodontic infections.

Keywords

Origanum vulgare; Rosmarinus officinalis; Essential oils; Enterococcus faecalis; Root canal disinfection; Antimicrobial activity.

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