Agropastoralis Scientia
Volume 1, Numéro 2, Pages 73-83
2025-01-28
Resistance Of Bradyrhizobium Sp. (lotus) To Metallic Stress
Authors : Degaichia Hoceme . Boussahoua Ahmed Ramzi . Bakria Touati .
Abstract
The objective of this study is to characterize the bacterial strain Bradyrhizobium sp. (Lotus) and to determine its resistance to trace metal elements (TM) such as copper (Cu(II)) and cadmium (Cd(II). Bradyrhizobium sp. (Lotus) was isolated from nodulated plants of Lotus ornithopodioïdes. Different characteristics of the strain were evaluated, such as growth, bacterial mobility, enzyme and polysaccharide production. The minimum inhibitory concentration (MIC) of TM was determined in vitro and in silico by measuring the optical density at a specific wavelength. The isolate is identified as Bradyrhizobium sp. (Lotus ), which is a Plant Growth Stress Regulating Bacteria (PGSRB) Gram-negative and slow growing, producing succinoglycan, mobile, possessing urease activity. The isolate produces Indole Acetic Acid (IAA) at 70.6 µg/ml. Bradyrhizobium sp. (Lotus) showed more pronounced resistance to Cd(II) compared to Cu(II). The MIC of Cd(II) was estimated in silico to be approximately 10 gL -1 , while the MIC of Cu(II) was 2 gL -1 . Statistical analyzes confirmed a significant relationship between the concentration of TM and the number of bacterial cells. This study made it possible to characterize the bacterial strain Bradyrhizobium sp. (Lotus) and determine its resistance to TM. The results suggest that this strain can be used in remediation or bioremediation applications involving environments contaminated with heavy metals such as copper and cadmium
Keywords
Lotus ornithopodoides L. ; Heavy metals ; Phytoremediation ; Rhizobacteria ; PGPR
Les articles similaires
Bouchenak Fatima
.
pages 1255-1266.
محمد بلهادف
.
مصطفى بوزازوة
.
ص 199-226.
Derwich, & E
.
Benziane Z
.
Benaabidate L
.
pages 79-88.
Gaëlle Saladin
.
pages 39-48.
Panaetov V. P.
.
pages 20-23.
